Blue pippin
In the first of the six runs, involving 13 samples, we used a Blue Pippin instrument to size-select DNA amplicons before sequencing. This was done to separate microbiome from host mitochondrial 16S rRNA gene sequences. This method proved untenable due to Blue Pippin performance inconsistencies and we did not apply physical size-selection to the ...Clean-lined Pippin comes with a tailored, zip-on, zip-off cover that gives the chair its sophisticated personality. Pippin comes in NaughtOne's wide selection of fabrics and finishes, and it's also available with or without the handle and castors. While this lounge chair is built for longevity, it also considers the circular economy.Libraries were size-selected using Blue Pippin system with 2% gel Blue Pippin Cassette (Sage Science, United States) with a range of 200-600 bp and their quality and quantity was assessed using Agilent DNA kit (Agilent technologies, United States). Libraries were pooled and sequenced using a NextSeq 500/550 High Output Kit v2.5 (300 cycles ...
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1. Blue Pippin Start up 1.1. Powering up the Blue Pippin. The Blue Pippin instrument has to be rebooted each time it is used in order to connect to the server. Once it is turned off it loses the connection. Either reboot or if the device is turned off prior to use, locate the black power button at the rear of Blue Pippin. Push the button and ... The BluePippin is a preparative electrophoresis platform that separates and extracts DNA or protein from agarose. Using pre-cast gel cassettes, biomolecules are automatically collected in buffer according to software-input target ranges. The BluePippin also features pulsed-field electrophoresis power which enable accurate collection of kb-sized DNA. Cassettes are available for DNA size ... selected using the Blue Pippin (Sage Science, United Kingdom). Illumina libraries were constructed with a PCR-free method. using NEBNext End Repair, NEBNext dA-t ailing and Blunt T/A.
Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes. To resolve very large DNA fragments, pulsed-field runs may last several hours. View the Blue Pippin cassette reference chart to explore the DNA size selection options and run times.The Blue Pippin also does your size selection, so you don't burn out pores sequencing lots of little stuff. This should help yield too. Then it's all down to the library prep, and @gringer's answer has some good tips on that. Share. Improve this answer. FollowSpecifications. Concentration. 0.75%. Product Type. Agarose Gel Cassette. Quantity. 10 Pack. Provide Content Correction. Sage Science™ BluePippin™ Dye Free 0.75% Agarose Gel Cassettes are disposable and pre-cast cassettes with agarose and running buffer. Libraries were size-selected on Blue Pippin (Sage Science, Beverly, MA, USA) instrument using marker S1, with 7 and 10 kb lower cutoff. Size selected DNA was collected and purified using AMPure PB beads (Beckman Coulter, Indianapolis, IN, USA). Sequencing primer was annealed and DNA polymerase bound to the SMRT bell templates. The libraries ...BluePippin Operations Manual 460013 Rev E 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.
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Page 92 978-922-1832 www.sagescience.com [email protected] 500 Cummings Center Suite 2400 Beverly, MA 01915 25-15 BluePippin Operations Manual 460013 Rev F... View and Download Sage Science BluePippin operation manual online. DNA Size Selection System.The Blue Pippin applies pulsed-field electrophoresis technology to size select DNA for downstream applications (i.e. library preparation). This machine is extremely beneficial for high molecular weight applications due to its high pass filter that allows you to collect all fragments above a specified threshold.
BluePippin and Pippin HT. The Pippin systems from SageScience allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. Our Core runs both the BluePippin and the Pippin HT.BluePippin Prep. The Pippin Prep is an electrophoresis platform capable of size-selecting DNA fragments and automatically collects the fractions in buffer according to the user-specified size range. It is routinely used to size-select next generation sequencing libraries. It is capable of performing narrow size selections from 50bp to 50kb in ...
ksu basketball radio Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length). can am renegade 850 top speedonline teaching games free The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy. We ask that you consider turning off your ad blocker so ... learning styles in the classroom BLU0001 The BluePippin System includes instrument, monitor, keyboard, and mouse. Dimensions are 7"H X 11"W X 21"D, and weight is 15 lbs. Requires 100-240 VAC. commercialization processku basketball listen livenew driver checklist The gene structure of Pfpα is shown with exons in blue boxes and introns with a black line. ... and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries ...8 AFA-TUBE™ TPX Strip (PN 520292) Resource Type: Product Inserts and User Manuals Download PDF douglas county kansas health department Page 6 P/N 100-286-000-07 STEP Purify DNA Notes 1 Add 0.45X volume of AMPure PB beads to the End-Repair reaction. (For detailed instructions on AMPure PB bead purification, see the Concentrate DNA section.) 2 Mix the bead/DNA solution thoroughly. 3 Quickly spin down the tube (for 1 second) to collect the beads. Do not pellet beads.selection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq). delario robinsonbest paladin race wotlkosrs melee weapons From the genomic DNA, 20 Kb insert size libraries were prepared and size selected using Blue Pippin™ and sequenced using C3 in combination with P5 polymerase chemistry for 2 SMRT cells per genome with 180 min or longer movie time and stage start.