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We have had some difficulty when running GBS pools on the Pippin Prep. The pools they produce are at times showing a lack of abundance. We are running this experiment to gain insiThe Paenibacillus sp. strain B-LR circular genome is 6,694,765 bp long with a 46.76% GC content and contains 6,129 coding sequences (CDSs), 81 tRNAs, and 30 rRNAs. The sequence was submitted to antiSMASH analysis of secondary metabolite biosynthesis gene clusters ( 8 ). Among the 42 clusters identified, 18 were only putative, whereas 25 were ...For the MinIon and PromethIon sequencers from Oxford Nanopore, the company recommends BluePippin sizing for various protocols. This library prep workflow for both sequencing systems uses BluePippin to eliminate shorter fragments; one example of outcomes shows a whopping 255 Kb read from an E. coli experiment. There's a similar rationale for ...Illumina paired-end library construction is the most popular use of the Pippin platform, and this is a typical citation. We are proud to have our products cited in the context of great research. Mate-pair sequencing: Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencingBlue Pippin Size Selection. Blue Pippin Sample Submission Form. Blue Pippin Submission Template; Library Preparation. We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.Combine 30 μl of cleaned PCR product with 10 μl of V1 maker (from the Blue Pippin Cassette Kit), mix and load the mixture on 2% (wt/vol) agarose gel cartridge (Sage Science). 71.Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.Selection Chart Pippin Prep BluePippin PippinHT Max. DNA input 5 μg 5 μg 1.5 μg Max. Sample Capacity 5 5 24 Pulsed-Field No Yes Yes Run Times (100 bp - 1.5 kb) 50-90 min 50-90 min* 25-45 min* Size Selection Range 100 bp - 1.5 kb 100 bp - 50 kb 100 bp - 1.5 kb High-Pass Size Filtering No Yes Yes Low-Pass Size Filtering Yes Accuracy > 93% ...Page 5 Experimental Protocol STEP Preparing RiboMinus Magnetic Beads Notes 1 Resuspend the RiboMinus Magnetic Beads in its bottle by thoroughly vortexing. 2 For each sample, pipet 250 μL of the bead suspension into a sterile, RNase-free, 1.5 mL LoBind tube. 3 Place the tube/s with the bead suspension on a magnetic rack for 1 minute. Gently aspirate and discard the supernatant.1. Blue Pippin Start up 1.1. Powering up the Blue Pippin. The Blue Pippin instrument has to be rebooted each time it is used in order to connect to the server. Once it is turned off it loses the connection. Either reboot or if the device is turned off prior to use, locate the black power button at the rear of Blue Pippin. Push the button and ... BN124141 Pippin Product Line Brochure (4 pages): Flyers: BN0205147. Paired-end sequencing flyer. for Illumina platforms. BN0205148. Pippin Pulse flyer. Pulsed-Field for gel boxes. BN0205149.In our Iso-Seq analysis, we detected a total of 27,860 transcripts, of which 11,342 (40.7%) are novel. Furthermore, 7,065 (25.4%) were identified as full-length, that is, the starting exon of an ...Are you looking for health insurance? Blue Cross insurance is one provider option that is widely available and, therefore, is likely to come up in your search. Learn more about where and how Blue Cross operates, including information on wha...为了对它们的生态学和进化有更多的了解，我们对三株从抑制病害土壤中分离出来的有很好抑菌效果的链霉菌菌株进行了全基因组测序，并将它们与之前测序的菌株进行比较。. 文章展示了从头测序（de novo sequencing）获得的3个链霉菌菌株的高质量全基因组 ...(B) The final indexed PCR products were purified using Ampure XP beads using BlueCat BlueWasher, pooled into a single library using Hamilton Starlet, and size selected using Sage Science Blue Pippin.

It was then centrifuged at 5000 × g for 10 min at 4°. The solution was then purified, washed, and eluted using the Qiagen genome-tip kit instructions. Sequencing libraries were created by shearing DNA to 35 kb on a Megaruptor (Diagenode) and selecting for 18-50 kb using a Blue-Pippin (Blue Pippin system, Sage Science, Beverly, MA, USA).Feb 1, 2022 · The importance of structural variants (SVs) for human phenotypes and diseases is now recognized. Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed, few benchmarking procedures are available to confidently assess their performances in biological and clinical research. The bacterial wilt of peanut (Arachis hypogaea L.) caused by Ralstonia solanacearum is a devastating soil-borne disease that seriously restricted the world peanut production. However, the molecular mechanism of R. solanacearum-peanut interaction remains largely unknown. We found that R. solanacearum HA4-1 and PeaFJ1 isolated from peanut plants showed different pathogenicity by inoculating ...lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed using

The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ...Pippin Gel Cassettes. Gel Cassettes are pre-cast with agarose, and use ethidium bromide to visualize DNA and provide more uniform migration of fragments. Cassettes have a capacity to run 4 samples and are shipped in boxes of 10 each. Loading dye and DNA marker (for calibration) sufficient for all 40 runs are provided with each shipment. …

Reader Q&A - also see RECOMMENDED ARTICLES & FAQs. 8 thg 10, 2023 ... Blue, Yellow, Magenta, Cyan. Transpa. Possible cause: Find compatible pollenizers for Blue Pearmain apple trees, based on flowering grou.